FIG. 3.
Murine CD44 splicing patterns. (A) CD44 was amplified from normal mouse tissue cDNAs and, following Southern transfer, probed with an oligonucleotide which detects all CD44 isoforms (panel 1). A total of 1,000 to 2,000 CD44-containing clones from each tissue were analyzed. The majority of these clones were CD44H, containing no alternative exons (panel 2). The exon compositions of 328 alternative exon-containing variants were determined (panels 3 to 8) (liver [n = 28], kidney [n = 77], spleen [n = 4], colon [n = 80], small (Sm) intestine (Intest) [n = 51], and stomach [n = 88]). No alternatively spliced isoforms were observed in skeletal (Sk) muscle. (B) Schematic of the alternative exon compositions of alternatively spliced cDNA clones included in this study. The numbers at the left represent alternative exons V1 to V10. Exons scored as present are indicated by black dots.