FIG. 5.
Western blot displaying the PB-dependent binding of mCAR1 and RXR to NR1. Liver nuclear extracts (1 mg of proteins in 1 ml of the incubation mixture) from the PB-treated mice at each time point (1 to 24 h) were incubated with NR1 affinity beads. In the right-hand lane (6 hr. + NR1), an excess amount of NR1 oligonucleotide (10 μg) was included as a competitor during the incubation. A 30-μl volume of the 100-μl eluates with 0.5 M NaCl was subjected to Western blot analysis with anti-RXRα IgG (1:3,000 dilution) and anti-CAR (peptide) serum (1:250 dilution). The results are representative of five or more independent experiments.