TABLE 1.
MHC-positive cellsa
| Expt | No. MHC positive/total no. (%) in cell line:
|
||||
|---|---|---|---|---|---|
| C2C12 | B1-B9 (RB-HBP1) | Con 1 | Con 2 | Con 3 | |
| 1 | 33/127 (26.0) | 32/225 (14.2) | 1/207 (0.48) | 2/321 (0.62) | 7/217 (3.2) |
| 2 | 30/96 (31.3) | 34/219 (15.5) | 1/194 (0.52) | 0/426 (0) | 5/207 (2.4) |
a
RB-expressing B1-B9 and three individual control lines with both selection markers were grown for 5 days in differentiation media. MHC-positive cells were detected by immunofluorescence and were quantitated from duplicate plates. Con, control.