FIG. 1.
Time course processing inhibition assay. P. falciparum 3D7 schizonts were incubated at 37°C with either adjuvant control (1:10 dilution) or postimmune serum at a 1:2,500 or 1:10 dilution, and samples were harvested at four time points as follows: T0 = 0 h, 0% rupture; T1 = 3 h, ∼30%; T2 = 4 h, ∼50%; T3 = 5.5 h, ∼70%; T4 = 7 h, >90% rupture. The samples were run on SDS-PAGE and Western blotted. The blot was probed with a mixture of two biotinylated primary antibodies: rabbit polyclonal anti-AMA-1 IgG and rat MAb 28G8dc1. Positions of molecular mass marker bands (Multimark; Invitrogen) are represented in kilodaltons on the left, and positions of the PfAMA-1-specific bands are shown on the right. Lane C had an equivalent number of uninfected erythrocytes incubated with a 1:10 dilution of the immune serum. NS, nonspecific bands derived from erythrocyte or serum components.