FIG. 4.
Western blots demonstrating reactivity of pilins of N. gonorrhoeae with MAb TEPC-15 against ChoP. The top and bottom are photographs of the same Western blot developed sequentially. The blot was first developed by using an anti-ChoP antibody (top). After recording the reactivity shown at the top, we cut the lanes in half and reacted the nitrocellulose strips marked with asterisks with MAb SM1 against class I pilins. After development, the strips were combined and rephotographed (bottom composite blot) to show colocation of the ChoP epitope (unmarked strips) and pilins (strips marked with asterisks). Lanes: 1, purified pili of N. meningitidis C311 as a control; 2 to 6, piliated variants of gonococcal strain MS11; 7 and 8, piliated gonococcal strains R10 and SU95; 9, molecular size markers. Size markers of 30 kDa (large arrowheads) and 21.5 kDa (small arrowheads) are shown. Note that the pilins of one variant of strain MS11 (lane 3) do not contain the ChoP epitope.