FIG. 5.
ICAM-1 inhibits the binding of 125I-labeled fimbriae to mouse peritoneal macrophages. 125I-labeled fimbriae (1 μg of protein) were treated or not treated with ICAM-1 (A) or laminin (B) at the indicated doses in each well of a microculture plate containing formalin-fixed peritoneal macrophages. The binding of 125I-labeled fimbriae to cells was measured after 4 h. Each assay was carried out in triplicate. The results are expressed as the mean ± SD percent inhibition of binding of 125I-labeled fimbriae (40,050 ± 2,171 cpm) in the absence of test samples. An identical experiment performed independently gave similar results.