FIG. 6.
(A) Colocalization of TCoB with tubulin was observed during mitosis. Methanol-fixed Vero cells probed with antibodies against TCoB and α-tubulin are shown. The right panel shows the indicated region of the left panel at 300% digital magnification. The left panel was acquired with a ×63 objective. Bar, 10 μM. (B) SKBR3 cells were transfected with RFP-TCoB (red), and MTOCs were analyzed by confocal microscopy using antibody against γ-tubulin staining (green). (C) Breast cancer MCF-7, SKBR3, and MCF-7 cells stably overexpressing HER2 were transfected with different TCoB and Pak1 plasmids or with TCoB- or Pak1-specific siRNA as indicated, and MTOCs were analyzed by γ-tubulin staining. Only quantitation of MTOCs in RFP-TCoB-expressing breast cancer cells was shown.