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Southern blotting experiments using scpA and the ORF as probes. Chromosomal material from different S. pyogenes strains were digested with XbaI, separated by agarose gel electrophoresis, and blotted to Hybond-N membranes. Probes were generated by radiolabeling of PCR products generated by the primer pair 1474-1476 to detect scpA and by the primer pairs 3244-3267 to detect the ORF sequence. AP1 chromosomal DNA was used as target to generate the PCR products.
