FIG. 1.
Coselection of adhesive phenotypes on RBC infected with P. falciparum. (A) Derivation of lines and clones from the FCR3S strain. FCR3S1 was cloned by limiting dilution, subjected to long-term culture, and repeatedly enriched for the rosetting phenotype. Clones FCR3S1.2 and FCR3S1.6 were derived by micromanipulation from rosetting and nonrosetting parasites, respectively. The line FCR3S/a was obtained by enrichment of nonrosetting parasites. The lines FCR3S/b and FCR3S1/b were generated by five consecutive rounds of panning on C32 melanoma cells. (B) Binding phenotypes of parasites derived from FCR3S. (a to c) Rosetting of uninfected RBC and autoagglutination of parasitized RBC. PRBC are stained with acridine orange and visualized by UV microscopy. (d to f) Cytoadherence to unfixed HUVEC. Cells were stained with Giemsa and examined by light microscopy. All magnifications, ×400.