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Strategy to entrap IS elements from ATCC 17616. (A) Structure of pGEN500. The arrows indicate the primer-annealing regions. F1 to F4 and R2 to R5 denote primers listed in Table 2. (B) The experimental scheme developed and used in this study. See the text for the details of this scheme. The pGEN500::IS derivative is indicated as pGEN500*. Tc, tetracycline.
