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Competition IFAa
| Antibody | Antigen | Antigen concn (μg/ml) resulting in:
|
||
|---|---|---|---|---|
| 50% inhibition | 100% inhibition | No inhibition | ||
| Fraction 2 | R0 | 10 | >100 | |
| Fraction 3 | R0 | ND | 0.1 | |
| Fraction 4 | R0 | ND | ND | 100 |
| Fraction 2 | R2 | ND | ND | 100 |
| Fraction 3 | R2 | <0.1 | 1 | |
| Fraction 4 | R2 | 0.1 | 10 | |
Soluble antigen was added to the three fractions at an antibody concentration which was fourfold higher than the IFA endpoint titer. ND, not determined.
