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Purification of M. thermoautotrophicum SerRS
| Fraction | Total amt of protein (mg) | Sp act (pmol min−1 mg−1) | Total activity (pmol) | Enrichment factor (fold) | Yield (%) |
|---|---|---|---|---|---|
| S-100 | 307.4 | 7.6 | 2,334 | ||
| Q-Sepharose | 44.1 | 43.3 | 1,905 | 5.7 | 81.6 |
| Phenyl-Sepharose | 4.62 | 109 | 504 | 14.3 | 21.6 |
| MonoQ 5/5 | 0.61 | 417 | 254 | 55 | 10.9 |
| Superose12 | 0.016 | 6,530 | 105 | 859 | 4.5 |
