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Polyacrylamide gel analysis of products under CCA assay conditions with yeast tRNA as the acceptor and [α-32P]ATP (A) or [α-32P]CTP (B) as the substrate. Lanes: 1, E. coli PAP I; 2, protein extract from BL21(DE3) with the pET11a expression vector; 3, E. coli tRNA nucleotidyltransferase; 4, B. subtilis PapS.
