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. 2023 Dec 19;14:1247592. doi: 10.3389/fimmu.2023.1247592

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Copyright © 2023 Martín Monreal, Kvist-Hansen, Massarenti, Steffensen, Loft, Hansen, Ødum, Skov and Nielsen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circulating NET levels and composition. Sera from 20 healthy donors (HDs) and 20 patients with psoriasis (PSO pts) were added to ELISA plates coated with an anti-MPO antibody to capture NETs. Subsequently, antibodies against (A) DNA, (B) IgG, (C) native LL37 (LL37) and (D) citrullinated LL37 (citLL37) were used as detection antibodies in combination with HRP-conjugated secondary antibodies, and optical density (OD) was measured at 450 and 540 nm. Dotted lines represent the cut-off between positive and negative values, as calculated by means of a regression model with segmented relationships. Red symbols represent values above the cut-off, and filled, black symbols represent subjects who tested negative in (A) and therefore were regarded as false positives.