Figure 1.
Overexpression of CD73 is correlated with CRC progression. (A) CD73 gene expression was compared between TCGA-COAD tumor samples and GTEx normal colon tissues. The analysis was performed online in the TCGA TARGET GTEx cohort in Xena platform (http://xena.ucsc.edu/). (B) tSNE scatter showed distribution and expression level of CD73 in epithelial cells including tumor (EpiT) and non-tumor (Epi) cells in a single cell sequencing dataset. (C) WB assays showed the expression of CD73 in eight pairs of CRC tissues (T) and adjacent non-tumor tissues (N). (D) IHC assays showed the expression of CD73 in CRC tissues (Tumor) and adjacent normal mucosas (Normal) of two representative cases. Bars in the right panel represent the percentage of patients with high or low CD73 expression. The scale bar represents 50 μm. (E) IHC assays showed the expression of CD73 in CRC tissues with or without metastasis. Bars in the right panel represent the percentage of patients with high or low CD73 expression. The scale bar represents 100 μm. (F) The expression of CD73 in stage I-IV CRC samples from the GSE39582 dataset. (G) The expression of CD73 in CRC samples with different statuses of mismatch repair (MMR) in the GSE39582 dataset. (H) The Kaplan-Meier survival curve shows the correlation between CD73 expression and overall survival (OS) in CRC patients from the GSE39582 dataset. Data in A, F-H are presented as means ± SD and analyzed using unpaired two-tailed Mann-Whitney U-test. * p < 0.05, ** p < 0.01, ***p < 0.001.