Figure 6: Capturing cell migration with SWIP.
(A) A still image from a timelapsed intravital imaging movie of the pancreas in an orthotopically injected mouse model of PDAC. (B) Still images from Supplemental Video S1 showing an example of tumor cells undergoing collective migration (yellow arrow). (C) Still images from Supplemental Video S2 showing examples of single-cell migration of a tumor cell (yellow arrow) and a macrophage (red arrow). Green = Dendra2-labeled tumor cells, Blue = CFP-labeled macrophages, Red = 155 kDa tetramethylrhodamine dextran-labeled blood serum. Scale bars = 50 μm (A), 15 μm (B,C). Abbreviations: SWIP = stabilized window for intravital imaging of the pancreas; CFP = cyan fluorescent protein; PDAC = pancreatic adenocarcinoma.