Figure 4: Microcartography used for serial imaging to relocalize the same areas of interest within the optical window.
Intravital imaging of a single region of murine pancreas, showing the same lobule relocated over 2 consecutive days (D1-D2) using microcartography. Yellow dashed lines outline the boundaries of the same lobule. The solid lines highlight the same blood vessels (as evidenced by the presence of red labeled blood serum within the lumen) identified each consecutive day. Red = 155 kDa tetramethylrhodamine dextran-labeled blood serum, Cyan = CFP labeled pancreatic cells (MMTV-iCre/CAG-CAC-ECFP transgenic mice) (Inset) Image of the SWIP with scratches for microcartography. The use of microcartography to relocate regions of interest during serial imaging is permitted by the three etched lines on the window frame (inset). Scale bars = 50 μm.