Skip to main content
. Author manuscript; available in PMC: 2024 Oct 6.
Published in final edited form as: J Vis Exp. 2023 Oct 6;(200):10.3791/65498. doi: 10.3791/65498

Figure 2: Overview of the surgery protocol of orthotopic injection of pancreatic ductal adenocarcinoma cells (KPC).

Figure 2:

(A) Illustration of how to hold surgical forceps. (B) Illustration of how to hold Castroviejo scissors. (C) Illustration of how to hold the vacuum pickup tool. (D) Sanitizing the skin. (E) Incision in the skin. (F) Incision in the muscle. (G) Splayed pancreas. (H) Insertion of needle into the desired site of injection. (I) Injection of cancer cell suspension creating a bubble (blue arrow). (J) Pancreas returned to peritoneal cavity. (K,L) Closing of the muscle layer with an interrupted silk suture. (M,N) Closing of the skin with an interrupted silk suture. (O) Cyanoacrylate glue applied to incision.