FIG. 7.
Western blot analysis of the complementation of the E. coli csg mutants. Results for whole-cell preparations treated with formic acid are shown. The mutant (−) and the mutant harboring the respective complementing plasmid (+) were run next to each other. Except for MHR480 (ΔcsgE3), none of the E. coli mutants showed a CsgA signal derived from polymerized fibers. MHR261 (csgB2) and MHR503 (csgD6) were complemented to wild-type levels by using plasmids with the single genes. MHR426 (csgF4) was complemented to wild type only when a plasmid carrying csgEFG was used. Faint signals of CsgA were detected when pCSGA was introduced into MHR204 (csgA::Tn105).