TABLE 1.
Plasmids and bacterial strains used in this study
| Bacterial strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa PAO1 | Wild type | G. Pier, Harvard University |
| E. coli | ||
| LE392 | Wild type | 17 |
| SURE | lacIqZΔM15, Tcr | Stratagene |
| RO138 | lpxA temperature-sensitive mutant, recA | 21 |
| Plasmids | ||
| Cosmid 1137 | lpxA+ cosmid clone in pCP13, Tcr | 17, this study |
| pBluescript KS II | lacZ, Ampr | Stratagene |
| pGD2 | 3.0-kb PstI fragment from cosmid 1137 cloned into pBluescript, lpxA+ Ampr | This study |
| pGD3 | 1.7-kb SalI deletion from pGD2, lpxA+ Ampr | This study |