TABLE 4.
Gradient plate sensitivity tests
| Strain | Genotype | Growth (mm across gradient)a on agar containing:
|
||||
|---|---|---|---|---|---|---|
| Nitrofurantoin (0 → 10 μg/ml) | Bleomycin (0 → 0.57 μg/ml) | Mitomycin (0 → 1.7 μg/ml) | MMS (0 → 0.83 μg/ml) | tert-Bu(OH)2 (0 → 0.33 μg/ml) | ||
| AB1157 | Wild type | 71, 72 | 80, 80 | 70, 70 | 70, 70 | 80, 80 |
| BW1161 | nfi | 50, 46 | 68, 65 | 70, 60 | 75, 75 | 80, 80 |
| BW9109 | Δxth | 44, 44 | 35, 50 | |||
| BW1166 | Δxth nfi | 40, 40 | 35, 50 | |||
| RPC500 | nfo | 42, 40 | 55, 55 | 35, 55 | 70, 70 | |
| BW1162 | nfo nfi | 38, 38 | 35, 35 | 35, 53 | 70, 65 | |
a
To minimize the effects of the gradient variation, both within and between plates, all the strains tested against a given agent were applied to a single plate as duplicate samples in a staggered array. The test for nitrofurantoin sensitivity was an exception in that two plates were used for each experiment, both of which contained AB1157 and BW1161 for reference. Each number is an average of results for duplicate samples. The results are presented in the following format: experiment 1, experiment 2. MMS, methyl methanesulfonate; tert-Bu(OH)2, tert-butyl hydroperoxide.