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Time course of the reduction in c-Myc protein levels by pSilencer–c-Myc. Exponentially proliferating MCF-7 cells were transfected with pSilencer–c-Myc or pSilencer and whole cell lysates were prepared at the time points indicated. Total cell lysates were separated by SDS–polyacrylamide-gel electrophoresis and immunoblotted with an antibody against c-Myc; expression levels were normalized for loading by probing for β-actin.
