FIG. 2.
ICP27 deletion, truncation, and substitution mutants. (A) A schematic representation of the ICP27 512-amino-acid protein shows the leucine-rich region (LRR), the NLS, the RGG box RNA binding domain, three predicted KH motifs, and a zinc finger-like domain (CCHC). The positions of the mutations are shown. (B) The residues that are deleted or altered are indicated. The ability of the mutant viruses to replicate on noncomplementing cells is indicated relative to that of the wild-type virus, with ++++ indicating nearly wild-type levels and − indicating that these mutants require an ICP27-complementing cell line to replicate. tsLG4 fails to replicate at a nonpermissive temperature. Ref., reference.