TABLE 2.
Rifamycin MICs for clinical isolates of M. tuberculosis with mutant rpoB alleles
| Clinical isolate | Affected amino acid(s) (change)b | MIC (μg/ml) ofa:
|
|||
|---|---|---|---|---|---|
| Rifam- pin | Rifa- butin | Rifa- pentine | KRM1648 | ||
| Resistantc | |||||
| TB54 | 511 (Leu→Pro) | 2 | 0.5 | 1 | <0.01 |
| TB022 | 513 (Gln→Pro) | 32 | 8.0 | 32 | 0.32 |
| TB872 | 513 (Gln→Pro) | 32 | 4.0 | 32 | 0.32 |
| TB713 | 513 (Gln→Leu) | 16 | 2.0 | 16 | 0.16 |
| TBU36 | 514 (Phe insertion) | 32 | <2.0 | 32 | <0.16 |
| TB3908 | 516 (Asp→Val) | 32 | <0.5 | 32 | 0.01 |
| TBYE-67 | 516 (Asp→Val) | 32 | 0.5 | 32 | 0.01 |
| TBGro-1 | 516 (Asp→Tyr) | 2 | <0.5 | 8 | 0.04 |
| TB33 | 517 (Gln deletion) | 32 | 8.0 | 64 | 2.56 |
| TB4183 | 518 (Asn deletion) | 64 | 64 | >32 | 5.12 |
| TB053 | 518–519 (Asn deletion) | >64 | >32 | >32 | >10.24 |
| TBRos-1 | 519 (Asn→Lys) | 32 | 0.5 | 32 | 0.01 |
| TB3505 | 521 (Leu→Met) | 32 | 8.0 | 32 | 2.56 |
| TBNor-1 | 522 (Ser→Leu) | 32 | 0.5 | 32 | 0.01 |
| TB2230 | 522 (Ser→Leu), 516 (Asp→Val) | >32 | >1.0 | 32 | 0.12 |
| TBRR-1 | 526 (His→Arg) | 64 | 64 | <32 | 5.12 |
| TB5095 | 526 (His→Leu) | 16 | 8 | 16 | 0.56 |
| TBRR-7 | 526 (His→Pro) | >64 | 32 | >64 | 2.56 |
| TB160 | 526 (His→Pro) | >64 | >32 | >64 | 5.12 |
| TBYE-68 | 526 (His→Tyr) | >64 | 32 | >64 | >2.56 |
| TB3081 | 526 (His→Tyr) | >64 | >32 | >64 | 5.12 |
| TB052 | 526 (His→Asp) | >64 | 64 | >32 | >2.56 |
| TB3140 | 526 (His→Asp) | >64 | 64 | >32 | 5.12 |
| TB91 | 526 (His→Asp), 509 (Ser→Leu) | >64 | 8 | >64 | 0.64 |
| TB023 | 527 (Lys→Gln) | >32 | 16 | 32 | 2.56 |
| TBYE-14 | 531 (Ser→Leu) | >64 | 32 | >64 | 10.24 |
| TB3241 | 531 (Ser→Leu) | >64 | 32 | >64 | >10.24 |
| TB019 | 531 (Ser→Phe) | 64 | 32 | >64 | 10.24 |
| TB3126 | 533 (Leu→Pro) | 64 | 8 | 64 | 2.56 |
| Susceptible | |||||
| TBSmi-1 | None | 0.5 | <0.5 | <0.12 | <0.01 |
| TBH1-7 | None | 0.5 | <0.5 | 0.12 | <0.01 |
a
MICs determined by the BACTEC radiometric method.
b
Amino acid numbers correspond to the E. coli numbering system for the RNA polymerase β-subunit.
c
Clinical isolates of M. tuberculosis resistant to rifampin at ≥2 μg/ml by the BACTEC radiometric method or at 1 μg/ml by conventional agar plate testing.