Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1987 Sep;85(1):294–298. doi: 10.1104/pp.85.1.294

Hill Reaction, Hydrogen Peroxide Scavenging, and Ascorbate Peroxidase Activity of Mesophyll and Bundle Sheath Chloroplasts of NADP-Malic Enzyme Type C4 Species 1

Yoshiyuki Nakano 1,2, Gerald E Edwards 1
PMCID: PMC1054244  PMID: 16665673

Abstract

Intact mesophyll and bundle sheath chloroplasts wee isolated from the NADP-malic enzyme type C4 plants maize, sorghum (monocots), and Flaveria trinervia (dicot) using enzymic digestion and mechanical isolation techniques. Bundle sheath chloroplasts of this C4 subgroup tend to be agranal and were previously reported to be deficient in photosystem II activity. However, following injection of intact bundle sheath chloroplasts into hypotonic medium, thylakoids had high Hill reaction activity, similar to that of mesophyll chloroplasts with the Hill oxidants dichlorophenolindophenol, p-benzoquinone, and ferricyanide (approximately 200 to 300 micromoles O2 evolved per mg chlorophyll per hour). In comparison to that of mesophyll chloroplasts, the Hill reaction activity of bundle sheath chloroplasts of maize and sorghum was labile and lost activity during assay. Bundle sheath chloroplasts of maize also exhibited some capacity for 3-phosphoglycerate dependent O2 evolution (29 to 58 micromoles O2 evolved per milligram chlorophyll per hour). Both the mesophyll and bundle sheath chloroplasts were equally effective in light dependent scavenging of hydrogen peroxide. The results suggest that both chloroplast types have noncyclic electron transport and the enzymology to reduce hydrogen peroxide to water. The activities of ascorbate peroxidase from these chloroplast types was consistent with their capacity to scavenge hydrogen peroxide.

Full text

PDF
294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asada K., Kiso K., Yoshikawa K. Univalent reduction of molecular oxygen by spinach chloroplasts on illumination. J Biol Chem. 1974 Apr 10;249(7):2175–2181. [PubMed] [Google Scholar]
  3. Behrens P. W., Marsho T. V., Radmer R. J. Photosynthetic o(2) exchange kinetics in isolated soybean cells. Plant Physiol. 1982 Jul;70(1):179–185. doi: 10.1104/pp.70.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Canvin D. T., Berry J. A., Badger M. R., Fock H., Osmond C. B. Oxygen exchange in leaves in the light. Plant Physiol. 1980 Aug;66(2):302–307. doi: 10.1104/pp.66.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chapman K. S., Berry J. A., Hatch M. D. Photosynthetic metabolism in bundle sheath cells of the C4 species Zea mays: Sources of ATP and NADPH and the contribution of photosystem II. Arch Biochem Biophys. 1980 Jul;202(2):330–341. doi: 10.1016/0003-9861(80)90435-x. [DOI] [PubMed] [Google Scholar]
  7. Charles S. A., Halliwell B. Effect of hydrogen peroxide on spinach (Spinacia oleracea) chloroplast fructose bisphosphatase. Biochem J. 1980 Aug 1;189(2):373–376. doi: 10.1042/bj1890373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furbank R. T., Badger M. R., Osmond C. B. Photosynthetic oxygen exchange in isolated cells and chloroplasts of c(3) plants. Plant Physiol. 1982 Oct;70(4):927–931. doi: 10.1104/pp.70.4.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ghirardi M. L., Melis A. Localization of photosynthetic electron transport components in mesophyll and bundle sheath chloroplasts of Zea mays. Arch Biochem Biophys. 1983 Jul 1;224(1):19–28. doi: 10.1016/0003-9861(83)90186-8. [DOI] [PubMed] [Google Scholar]
  10. Hardt H., Kok B. Comparison of photosynthetic activities of spinach chloroplasts with those of corn mesophyll and corn bundle sheath tissue. Plant Physiol. 1978 Jul;62(1):59–63. doi: 10.1104/pp.62.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jablonski P. P., Anderson J. W. Light-dependent reduction of hydrogen peroxide by ruptured pea chloroplasts. Plant Physiol. 1982 Jun;69(6):1407–1413. doi: 10.1104/pp.69.6.1407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jenkins C. L., Boag S. Isolation of Bundle Sheath Cell Chloroplasts from the NADP-ME Type C(4) Plant Zea mays: Capacities for CO(2) Assimilation and Malate Decarboxylation. Plant Physiol. 1985 Sep;79(1):84–89. doi: 10.1104/pp.79.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kanai R., Edwards G. E. Separation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies. Plant Physiol. 1973 Jun;51(6):1133–1137. doi: 10.1104/pp.51.6.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marsho T. V., Behrens P. W. Photosynthetic oxygen reduction in isolated intact chloroplasts and cells in spinach. Plant Physiol. 1979 Oct;64(4):656–659. doi: 10.1104/pp.64.4.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Radmer R. J., Kok B. Photoreduction of O(2) Primes and Replaces CO(2) Assimilation. Plant Physiol. 1976 Sep;58(3):336–340. doi: 10.1104/pp.58.3.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Radmer R., Ollinger O. Light-driven Uptake of Oxygen, Carbon Dioxide, and Bicarbonate by the Green Alga Scenedesmus. Plant Physiol. 1980 Apr;65(4):723–729. doi: 10.1104/pp.65.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schuster G., Ohad I., Martineau B., Taylor W. C. Differentiation and development of bundle sheath and mesophyll thylakoids in maize. Thylakoid polypeptide composition, phosphorylation, and organization of photosystem II. J Biol Chem. 1985 Sep 25;260(21):11866–11873. [PubMed] [Google Scholar]
  18. Volk R. J., Jackson W. A. Photorespiratory phenomena in maize: oxygen uptake, isotope discrimination, and carbon dioxide efflux. Plant Physiol. 1972 Feb;49(2):218–223. doi: 10.1104/pp.49.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker G. H., Izawa S. Photosynthetic electron transport in isolated maize bundle sheath cells. Plant Physiol. 1979 Jan;63(1):133–138. doi: 10.1104/pp.63.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES