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. 2023 Sep 15;9:95. doi: 10.1038/s41421-023-00592-6

Fig. 8. SAH-mAH2-5 suppresses progression and enhances chemosensitivity of pancreatic cancer cells in PDO and PDOX models.

Fig. 8

a Generation of four PDOs and orthotopically injected PDOX. b Protein expression levels of USP10 and N1ICD in four PDOs detected by western blot. c–f Organoid area measured by microscopy in four PDOs treated with SAH-CTRL or various concentrations of SAH-mAH2-5. g–j Orthotopic tumor volume and survival analysis of four PDOX models treated with SAH-CTRL or various concentrations of SAH-mAH2-5. k–m Organoid area and apoptosis rate stained by Calcein-AM/PI and measured using flow cytometry of GEM-resistant PDAC-R incubated with GEM, SAH-mAH2-5, or a combination of both. n Orthotopic tumor volume and survival analysis of PDOX model injected with PDAC-R and treated with GEM, SAH-mAH2-5, or a combination of both. The data are presented as the mean ± SD of three independent experiments. ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student’s t-test (c–f, l, m). ns no significance; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA (g–j, n). ns, no significance; **P < 0.01, ****P < 0.0001 by log-rank test (n). Scale bars, 200 μm (k).