FIG. 4.
Western immunoblot analysis of mouse anti-rP44 serum using six different HGE agent isolates and the rP44 antigen. Samples subjected to SDS-PAGE consisted of 10 μg of purified whole-cell preparation of the HGE agent isolates 13, 3, 11, 2, 6, and USG and affinity-purified rP44. These proteins were transferred to a nitrocellulose sheet and incubated with a 1:1,000 dilution of antisera. The numbers on the left indicate molecular masses in kilodaltons based on the broad-range prestained standards (Bio-Rad).