FIG. 3.
(A) Detection of the PHMGCR-CAT-IRES-LUCIFERASE-(A)n transgene in Therese mice by Southern blotting. DNA was digested with the PvuII restriction enzyme and analyzed by Southern blotting. Lanes: 1, transgenic DNA; 2, nontransgenic DNA plus 100 copies of the pCJ9 plasmid; 3, nontransgenic DNA. Digestion of transgenic DNA with PvuII generated the expected fragments of 4,262 and 1,248 bp and an additional fragment of 3,499 bp due to the insertion of transgenes in tandem. (B) Schematic representation (not to scale) of the PHMGCR-CAT-IRES-LUCIFERASE-(A)n DNA fragment after PvuII digestion.