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Arabidopsis (Col-1 gl1) SREs identified after successive rounds of differential subtraction
| Round | Dot Blot | Sequenced | Unique Clones | Salt Regulated |
|---|---|---|---|---|
| First | 1,000 | 137 | 34 | 25 |
| Second | 6,000 | 157 | 91 | 42 |
| Third | 3,000 | 320 | 80 | 17 |
| Total | 10,000 | 614 | 205 | 84 |
Salt regulation was based on detection of altered steady-state transcript abundance after NaCl treatment.
