Extended Data Fig. 2. Genome-wide CRISPR–Cas9 screen and gene ontology analysis in glucose and uridine.
(a) Replicate plot and Pearson correlation (R) analysis of n = 2 replicate genome-wide CRISPR–Cas9 screens in glucose and uridine highlighting genes of de novo pyrimidine synthesis, glycolysis and the PPP. Data are shown as log2 TPM + 1, or log2 fold of day 7. Top: Data shown at the individual sgRNA level. Bottom: Data shown at the gene level. (b) Top 10 ontologies associated with the genes enriched and depleted in uridine relative to glucose. Only 8 terms scored for the analysis of essential genes in uridine. The complete gene ontology analysis is reported in the Supplementary Data Table 1.
