Figure 4.
Effects of nocodazole on the dynamics of aurora B-GFP. NRK cells stably expressing aurora B-GFP were treated with 10–20 μM nocodazole at prometaphase (A), early anaphase (B) or midanaphase (C). Images of aurora B-GFP were recorded immediately before (left) and after nocodazole treatment (right). Time elapsed since the addition of nocodazole is given in minutes at the upper right corner. Treatment with nocodazole had no effect on centromeric association of aurora B at prometaphase (A); however, it inhibited the dissociation of aurora B from centromeres at early anaphase (B), and caused the dispersion of aurora B that was localized along midzone microtubules during late anaphase (C). Bar, 10 μm.