FIG. 6.
EDG5 and EDG3 mediate activation of Rho, but EDG1, EDG3, and EDG5 do not mediate activation of Cdc42. (A and B) EDG cells and CHO cells were stimulated with S1P at various concentrations or IGF I (100 ng/ml) for 3 min. (C and D) EDG cells and Swiss 3T3 cells were stimulated with S1P (0.1 μM) or bradykinin (0.1 μM), respectively, for the indicated times. The amounts of GTP-bound Rho and Cdc42 were determined as described in Materials and Methods. A portion (1/20) of the cell lysate was subjected to Western analysis for evaluating the amounts of total Rho and Cdc42 in each sample. The data are means ± standard errors of three determinations. The results are representative of two or three experiments with similar results.