FIG. 2.
Immature DCs are not permissive to HCMV AD169. DCs were derived from peripheral blood monocytes after 7 days of differentiation in AIMV medium supplemented with GM-CSF and IL-13. Filled histograms represent labeling with specific antibody against the indicated surface marker; dotted lines represent staining with the control isotype antibody (A). MRC5 cells and immature DCs were not infected (n.i) or infected with HCMV AD169 (MOI of 3) for the indicated times p.i. Viral IE protein expression was detected by Western blotting using a mouse anti-IE MAb (B), and the matrix protein pp65 was visualized by intracellular staining with anti-pp65 antibody under a Leitz fluorescence microscope (C). Results are representative of two experiments.