Table 2.
Size and Cell Cycle Measurements in Wing Discs from dMyc-Overexpressing Animals
| dMyc Overexpression in Wing Disc Cells | |||||||
|---|---|---|---|---|---|---|---|
| Genotype | FSC (Ratio) | % G1 | FSC G1 | %S | FSC S | % G2 | FSC G2 |
| Act>GFP | 1.0 | 29.3 | 1.0 | 30.2 | 1.0 | 40.5 | 1.0 |
| Act>dMyc | 1.5 | 5.3 | 1.5 | 35.0 | 1.5 | 59.7 | 1.5 |
| Act>CycE | 0.9 | 2.8 | 1.0 | 65.6 | 1.0 | 31.6 | 1.0 |
| Act>Stg | 1.0 | 41.0 | 1.0 | 32.0 | 0.9 | 27.0 | 0.9 |
| Act>dMyc, Stg | 1.1 | 19.9 | 1.1 | 65.5 | 1.1 | 14.6 | 1.2 |
Forward scatter (FSC) values and cell cycle phasing in cells overexpressing dMyc under control of Act>Gal4. FSC ratios are the mean FSC height for the appropriate transgene (which is coexpressed with GFP) to that of the internal, GFP-negative control cells. FSC ratios of cells in each phase are also given. At least three independent experiments gave similar results; the values shown here are from the analysis of one experiment.