Table 1.

Phenotypes and Rescue of dmyc Mutants

Genotype	Viability (%) (n)	Bristle Area, Pixels (n)	Cell Area (μm²)	Wing Width (μm)	Wing Length (μm)
WT	85 (214)	1994 (25)	173	930 ± 20	1827 ± 40
dmyc^dm1/Y	99 (221)	nd	nd	nd	nd
Dmyc ^P0 /Y	83 (197)	1497 (36)	150^d	865 ± 40^d	1685 ± 40^d
dmyc^P1/Y	29 (81)	768 (18)	149^d	820 ± 40^d	1587 ± 60^d
dmyc^P0/Y + dMyc	nd	1881 (38)^b	182^c	896 ± 35^c,d	1805 ± 13^c
dmyc^P1/Y + dMyc	77 (186)^a	1155 (21)^b	nd	nd	nd

	Complementation
Genotype	Viability (%)	Bristles	(n)	Breakpoints
dmyc^P0/WT	88	+	(198)	-
dmyc^P0/Df(1)N8	0	−	(137)	3C1;3D6
dmyc^P0/Df(1)75e19	45	−	(151)	3C11:3E4;5E
dmyc^P0/Df(1) GA102	100	−/+	(200)	3D5;3F7–8
dmyc^P0/Df(1) HC244	100	+	(82)	3E8;4F11

Viability is expressed as a percentage of the expected number. Wings from dmyc mutant adults were examined for trichome density to determine cell size (area). The number of trichomes from a specific region next to the posterior crossvein was counted within a 0.05 mm square and converted to area/cell (μM²). There were 43 squares from dmyc^P0 male wings, 31 each from dymc^P1 and control male wings, and 34 from dymc^P0; C765>dMyc male wings. Wing width is the distance from the posterior margin (at vein 5) perpendicular to the anterior margin. For wing length, vein 3 was measured from its origin in the hinge to the distal wing tip. A total of 20 dmyc^P0, 20 dmyc^P1, 26 dmyc^P0; C765>dMyc, and 15 control male wings were analyzed. Rescue of specific dmyc phenotypes by overexpression of dMyc cDNA was carried out by three means (see footnotes a–c). Complementation tests of dmyc^P0 with X chromosome deficiencies were carried out by crossing dmyc^P0 animals to wild-type (WT) flies or to flies carrying the deficiencies noted, and dymc^P0/Df progeny were scored for viability. dymc is located at 3D5. (n), number of animals scored; nd, not determined.

Heat shock–induced (HS) dMyc transgene was used.

Scabrous Gal4 and UAS-dMyc used.

C765-Gal4 and UAS-dMyc used.

p ≤ .001 (reference = WT).