Figure 4.
Overexpression of dMyc in Wild-Type Wing Discs
(A) FSC plots showing the relative size of control cells versus dMyc-expressing wing disc cells. Left panel, cells are expressing dMyc in random cell clones using Act>Gal4. dMyc-expressing cells also express GFP (green); control, GFP-negative cells are from the same disc (red). Right panel, FSC plot of En>dMyc, GFP-expressing discs. Green trace, posterior cells coexpressing GFP and dMyc; red trace, control, non-GFP-expressing anterior cells.
(B) Rhodamine-phalloidin staining of actin in a normal wing disc, showing that anterior and posterior cells are very similar in size at this stage of development. Arrow points to the boundary between anterior and posterior cells. A, anterior; P, posterior.
(C) Actin staining of a wing disc expressing dMyc in posterior cells (right part of disc) under En>Gal4 control. Inset shows magnified detail of the A/P border, showing the large, dMyc-expressing cells in the posterior. These cells have a mean FSC value 16% larger than anterior control cells (see [A]).