FIG. 6.
Coomassie-blue stained polyacrylamide gel showing acetamide-induced expression of M. tuberculosis relA/spoT in M. smegmatis. The mid-log phase of the culture harboring pMtrel2 was induced with 2% acetamide. A 1-ml aliquot was taken out at the indicated time interval after induction. Equal amounts of the total cellular proteins were resolved by SDS–8% PAGE. Lane 1, marker; lanes 2 to 8, 0, 2, 4, 6, 8, 10, and 20 h, respectively, after induction; lane 9, total cellular protein of a saturated culture of a strain harboring empty vector, pAGAN90.