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. 2023 Apr 6;14:1929. doi: 10.1038/s41467-023-37516-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Tamoxifen induction scheme for analysis of subcutaneous tissue. b Representative images of subcutaneous tissue of tamoxifen-treated control and iCdh5-GNAQ^Q209L mutant mice stained for ERG (white), pERK (red), Ki67 (green), and merge. Scale bar: 50 μm. c ERG staining marks EC nuclei. Quantification of ERG⁺ cells normalized per subcutaneous tissue area. pERK⁺ and Ki67⁺ and double-positive endothelial cells were normalized to total EC number. iCdh5-GNAQ^Q209L (n = 9) and control littermates (n = 9), mean±SD, unpaired two-tailed Welch’s t-test. Mouse genotypes are indicated. d GNAQ^Q209L mutant patient-derived vascular tumor tissue was analyzed. e Representative images of neonatal human foreskin and patient-derived tissue (cutaneous vascular tumor) with somatic GNAQ p.Q209L mutation immuo-stained for UEAI (Ulex europaeus agglutinin-I) (brown). Scale bar: 50μm. f Representative images of neonatal human foreskin and patient-derived tissue with somatic GNAQ p.Q209L mutation labeled for UEAI (white), pERK (red), Ki67 (green), and merge with DAPI (blue). Scale bar: 50 μm. g Quantification of the UEAI⁺ vascular area as percentage of total field area, pERK⁺, Ki67⁺ and double-positive EC calculated as events per mm² of vascular area; human foreskin tissue from n = 5 different donors, and n = 1 patient-derived tumor tissue, mean±SD. Source data for (c,g) are provided as a Source Data file. Schematic in (a,d) created with Biorender.com.