Table 2.
Excerpt of the mcrA gene sequence alignment of methanogens in the binding sites of the newly designed mcrA_F3 primer.
| Acc. No. | Species |
mcrA_F3 Sequence CTTGAARMTCACTTCGGTGGWTC |
Product Size with Primer mcrA-Rev (bp) * |
|---|---|---|---|
| KC618341.1 | Methanopyrus sp. | ATGGAGACCCACTTCGGTGGATC | 271 |
| HQ896500.1 | Methanomassiliicoccus luminyensis | ATGGAAACCCACTTCGGTGGTTC | 271 |
| AF414042.1 | Methanopyrus kandleri | ATGGAGACGCACTTCGGAGGTTC | 271 |
| MH004454.1 | Methanosarcina mazei | CTTGAAGACCACTTCGGTGGGTC | 271 |
| AB288266.1 | Methanosarcina horonobensis | CTTGAAGACCACTTCGGTGGATC | 271 |
| AB288268.1 | Methanosarcina subterranea | CTTGAAGACCACTTCGGTGGGTC | 271 |
| U22242.1 | Methanolobus oregonensis | CTTGAGGACCACTTCGGTGGATC | n/a |
| EU715818.1 | Methanolobus zinderi | CTCGAAGACCACTTCGGTGGATC | 271 |
| U22243.1 | Methanolobus taylorii | CTCGAAGACCACTTTGGTGGATC | n/a |
| AB703629.1 | Methanolobus profundi | CTCGAAGACCACTTCGGTGGATC | n/a |
| U22245.1 | Methanolobus vulcani | CTCGAAGACCACTTCGGTGGATC | n/a |
| JN081865.1 | Methanocella conradii | CTCGAGGACCACTTCGGCGGCTC | n/a |
| KJ441441.1 | Methanocella sp. | CTCGAGGACCACTTCGGTGGCTC | n/a |
| AB300467.1 | Methanocella paludicola | CTCGAGGACCACTTCGGTGGCTC | n/a |
| AF414044.1 | Methanomicrobium mobile | ATGGAAGACCACTTCGGCGGTTC | n/a |
| MH004450.1 | Methanoculleus marisnigri | ATGGAGGACCACTTCGGCGGTTC | 271 |
| AB300784.1 | Methanoculleus palmolei | ATGGAGGACCACTTCGGCGGGTC | n/a |
| AB300787.1 | Methanoculleus bourgensis | ATGGAAGACCACTTCGGCGGGTC | 271 |
| AB300779.1 | Methanoculleus chikugoensis | ATGGAGGACCACTTCGGCGGTTC | 271 |
| DQ229160.1 | Methanogenium organophilum | ATGGAAGACCACTTCGGTGGTTC | 271 |
| DQ229161.1 | Methanogenium boonei | ATGGAAGACCACTTTGGCGGGTC | n/a |
| JQ917190.1 | Methanobacterium sp. | CTGGAAGACCACTTTGGCGGTTC | n/a |
| GU385700.1 | Methanobrevibacter smithii | CTTGAAACTCACTTCGGTGGATC | 271 |
| LK054628.1 | Methanobrevibacter oralis | CTCGAAACTCACTTCGGTGGATC | n/a |
| CP001719.1 | Methanobrevibacter ruminantium | CTTGAAACTCACTTCGGTGGTTC | 271 |
| KF214818.1 | Methanobrevibacter sp. D5/mcrA+ plasmid | CTTGAAACTCACTTCGGTGGATC | 271 |
| EU919431.1 | Methanobrevibacter gottschalkii | CTTGAAACTCACTTCGGTGGATC | 271 |
| KC865050.1 | Methanobrevibacter boviskoreani | CTTGAAACCCAATTCGGTGGATC | 271 |
| EU919432.1 | Methanobrevibacter woesei | CTTGAAACTCAATTCGGTGGATC | 271 |
| KC865051.1 | Methanobrevibacter wolinii | CTTGAAACTCAATTCGGTGGATC | 271 |
| AF414035.1 | Methanobrevibacter arboriphilus | CTTGAAACCCAATTCGGTGGTTC | n/a |
| HM802934.1 | Methanobacterium movens | TTAGAAACCCTCTTCGGTGGATC | n/a |
| AY289750.1 | Methanobacterium thermaggregans | CTCGAGGACCAGTTTGGTGGATC | n/a |
| AB300780.1 | Methanothermobacter wolfeii | CTGGAGGACCAGTTCGGAGGATC | 271 |
| AB523786.1 | Methanothermobacter tenebrarum | CTCGAGACACAATTTGGAGGATC | 271 |
| HQ283274.1 | Methanothermobacter crinale | CTTGAGACACAGTTCGGCGGATC | n/a |
| KP006500.1 | Methanobacterium aggregans | CTCGAGACCCAGTTCGGTGGATC | n/a |
| AY386125.1 | Methanobacterium aarhusense | TTAGAAACACAGTTCGGTGGATC | n/a |
| AF313806.1 | Methanobacterium bryantii | CTTGAAGATCAGTTCGGTGGATC | n/a |
| X07793.1 | Methanococcus voltae | TTAGAAGACCACTTCGGTGGCTC | 271 |
| AF414048.1 | Methanothermococcus thermolithotrophicus | TTAGAAGACCACTTCGGAGGTTC | 271 |
| AB703637.1 | Methanococcus maripaludis | TTAGAAGACCACTTCGGTGGATC | 271 |
| M16893.1 | Methanococcus vannielii | TTAGAAGACCACTTTGGTGGATC | 271 |
| AY354034.1 | Methanococcus aeolicus | TTGGAAGACCACTTTGGAGGTTC | 271 |
| FJ982887.1 | Methanosphaera sp. | TTAGAAGACCACTTCGGTGGATC | 271 |
| AF414047.1 | Methanosphaera stadtmanae | TTAGAAGACCACTTTGGTGGATC | 271 |
* n/a—not available due to incomplete mcrA sequence.